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Exactly about Gene Transfer and Genetic Recombination in Bacteria

13 febrero 2020 | No Comments | kubo

Exactly about Gene Transfer and Genetic Recombination in Bacteria

The following points highlight the 3 modes of gene transfer and recombination that is genetic germs. The modes are: 1. Transformation 2. Transduction 3. Bacterial Conjugation.

Mode no. 1. Transformation:

Historically, the breakthrough of change in germs preceded one other two modes of gene transfer. The experiments carried out by Frederick Griffith in 1928 suggested for the time that is first a gene-controlled character, viz. Development of capsule in pneumococci, might be used in a variety that is non­-capsulated of germs. The transformation experiments with pneumococci fundamentally generated a discovery that is equally significant genes are constructed with DNA.

In these experiments, Griffith utilized two strains of pneumococci (Streptococcus pneumoniae): one by having a polysaccharide capsule producing ‘smooth’ colonies (S-type) on agar dishes that has been pathogenic. One other stress ended up being without capsule creating ‘rough’ colonies (R-type) and had been non-pathogenic.

Once the capsulated living bacteria (S-bacteria) had been inserted into experimental animals, like laboratory mice, a substantial percentage regarding the mice passed away of pneumonia and live S-bacteria could be separated through the autopsied pets.

As soon as the living that is non-capsulated (R-bacteria) were likewise inserted into mice, they stayed unaffected and healthy.

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